- Invoke histogram in the primary console:
to get a histogram menu:
- Make the following selections:
- After clicking the OK button in the above menu,
we'll get the following menu to specify
how to draw a trellis graph:
Panmo remembers the settings of Panel Order, Strips?, Packet Size Bars?, and Abbreviation Threshold used last time. If you are actually running Panmo
to follow this tutorial step by step,
what you see on your computer might not be the same as this
screen shot.
Adjust the settings of these 4 parameters to be
the same as those shown here.
- We move Wellfrom the attributes column to the selected attributes column by
- Drag and drop, or
- Click the left mouse button on it and press the S key on the keyboard.
After we do that, this trellis menu will become:
Note that Well is printed on a light yellow background
because it is a categorical variable.
Numerical variables are printed on a light blue background.
Also note that the number of columns and the number of panels
are updated to 48 automatically because Well has 48 different categories.
- Adjust the settings of Columns and Rows to 12 and 4, respectively:
- Click the OK button in the above trellis menu to get
a plate view of DNA profiles,
which is essentially a trellis graph of histograms of DNA content.
This plot demonstrates nicely one features of HCS data:
There are a lot of outliers. Please take a look at Tutorial 2 for how to separate outliers from the "good" data.
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