Creating Plate Views of HCS Data
Data:
HeLa cells were first treated with taxol, nocodazole, or etoposide for 24 hours and cells in rows A and B were treated with EdU for 30 minutes.
Acknowledgement: The data set is from Molecular Probes. Special thanks to:
- Yih-Tai Chen
- Dani Hill
- Scott Clarke
- The Molecular Probes team working on Click-iT (TM) EdU HCS assays
Readout:
3 cellular parameters are monitored:- Channel 1: DNA
- Channel 2: Cyclin B1
- Channel 3: EdU
Plate Views
- How to create a plate view of cell numbers.
Start the tutorial or view this 57-second screencast, which contains extra materials than the static tutorial. - How to create a plate view of DNA profiles.
Start the tutorial or view its screencast (32 seconds). - How to create a plate view of scan images
Start the tutorial or view this 122-second screencast, which contains extra materials than the static tutorial. - How to create a plate view of composite images
Start the tutorial or view this 69-second screencast, which contains extra materials than the static tutorial.