There were 10 fields in each well in this experiment.
The HCS scanner took 1 image in each of the 10 fields
in each well for each channel.
We'll show how to create a plate view of the Field 1 images
of the 48 wells.- Invoke Browse in the primary console:
to get a browse menu:
- Select Field:
- After clicking the OK button in the above menu,
we'll get a barplot:
- We'll retrieve all the cells in Field 1 from this barplot.
First paint all the Field 1 cells red:
- Put the barplot in the subset mode:
- Move the cursor into the plotting area of the barplot
and click the left mouse button to pop up a color
selection menu:
- Select red by clicking the left mouse button
over the red rectangle:
- After clicking the OK button,
all the red cells will be retrieved out of the barplot
and put in the temporary console:
- Invoke Cell Plot in the temporary console
to get a cell plot menu:
- Make the following selections:
- Click the OK button to get a plate view of the 48 Field 1 images:
- If you would like to take a closer look at the Field 1 image
from a particular well, say B3, put this cell plot in
the trellis display mode:
- Move the cross cursor over the B3 panel and click the left
mouse button to get the following:
- If you would like to hide the nucleus outlines in the above
2 plots of scan images, put any one of the two in the
scan image mode:
- Move the cross cursor over the image area and click
the right mouse button to pop up a menu:
- After Hide Nucleus Outlines is selected, the above 2 plots of scan images will look like:
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