- Start with the DNA profile plot generated in Step 3 of Tutorial 4:
- Put the DNA profile plot in the zoom mode:
- Move the magnifying glass cursor to where it is in the above
plot, press and hold down the left mouse button, drag the
cursor right till the right side of the red rectangle is
close to 5.0e+5,
and release the left mouse
button. Now the above DNA profile plot will look like the
following:
The area covered by the red rectangle is the area we just
zoomed into and a new histogram is created based on the
data in that area:
- Histogram "Ocular 2" contains the "good" cells.
To access these cells, put "Ocular 2" in the subset mode
first:
- As all cells in "Ocular 2" are of the same color,
clicking the left mouse button over the plotting area
will extract all the cells in "Ocular 2" and put them
in the temporary console:
- Follow steps outlined in this tutorial to get the following plate view of DNA profiles:
Recall that columns 1, 2, & 3 were control,
columns 4, 5, & 6 were treated by taxol,
columns 7, 8, & 9 were treated by nocodazole, and
columns 10, 11, & 12 were treated by etoposide.
As a comparison, here is a plate view of DNA profiles with outliers included.
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