- Start with the DNA profile plot generated in Step 3 of Tutorial 4:
- Put the DNA profile plot in the zoom mode:
- Move the magnifying glass cursor to where it is in the above
plot, press and hold down the left mouse button, drag the
cursor all the way to the right, and release the left mouse
button. Now the above DNA profile plot will look like the
following:
The area covered by the red rectangle is the area we just
zoomed into and a new histogram is created based on the
data in that area:
- Paint cells in the 3 right-most rectangles different colors.
- Put the above histogram in the subset mode:
- Move the cursor into the plotting area of "Ocular 2"
and click the right mouse button to pop up a menu:
- After selecting Retrieve Displayed Data, all the displayed data in "Ocular 2" are taken out and
put in the temporary console:
The temporary console tells us there are only 8 cells in
"Ocular 2".
- Invoke Cell Plot in the temporary console and
make the following selections in the ensuing cell plot menu:
- Click the OK button to get the following cell plots:
Notice the 4 "nuclei" defined
by the poor image processing software in the above cell plot;
2 of them are the 2nd and the 3rd highest values
of ObjectTotalIntenCh1.
The highest ObjectTotalIntenCh1 value is from a fake "nucleus" (the red one) defined
by the poor image processing software in the above cell plot.
If you are running Panmo to follow this tutorial,
you can put these cell plots in the trellis display mode
and click the left mouse button over an image panel
to get a closer look at that image.
To simulate that functionality here in HTML,
you can click any image panels in the above 4 cell plots
to get a cell plot of a single panel and image.
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